The study entails the screening of the leaves of three medicinal plants; Barleria dinteri, Grewia flava and Jatropha lagarinthoides, for presence of phytochemicals, as well as possession of antioxidant and antibacterial properties. The leaf samples of the three medicinal plants were collected from Zebediela in Limpopo province, South Africa. The extracts of the dried leaf samples were screened for the presence of phytochemicals using standard chemical tests, as well as the possession of antioxidant and antibacterial properties using DPPH assay and modified bioautographic procedure, respectively. Phytochemical screening indicated the presence of alkaloids, flavonoids, saponins and tannins in all medicinal plants under study, whereas anthraquinones and reducing sugars were only present in Grewia flava and Jatropha lagarinthoides. The leaves of all three plants showed possession of free radical scavenging activity against DPPH (an indication of antioxidant properties), as well as growth inhibition against Escherichia coli, Enterococcus faecaelis, Staphylococcus aureus and Pseudomonas aeruginosa. The current study indicates the presence of important phytochemicals, antioxidant and antibacterial properties in the leaves of Barleria dinteri, Grewia flava and Jatropha lagarinthoides which support the plants usage in African traditional medicine.