Phytochemical and antinutrients analyses of Trametes betulina, T. cingulata, Meripilus sumstinei and Oxyporus populinus fruiting bodies were carried out. The phytochemical screening result shows the presence of sterols and triterpenes in large amounts, except in M. sumstinei. The quantity of alkaloids, saponins, flavonoids and carbohydrates varied in all samples. The antinutrients analysis revealed the presence of tannins (10.14±0.15 – 55.88±0.25mg/100g), phytates (4.12±0.20 – 27.00±0.03 mg/100g), hydrogen cyanide (6.13±0.03 – 13.54±0.10 mg/100g), soluble oxalates (7.04±0.09 – 70.4± 0.02 mg/100g), insoluble oxalates (52.70±0.09 – 98.56±0.25 mg/100g) and total oxalates (105.60±0.31 – 158.40±0.44 mg/100g). These corky and woody polypores may be inedible due to their fibrous nature; however, the permissible antinutrient levels and inherent phytochemicals may find medicinal and industrial relevance.