Plants are always been the sources of medicine from prehistoric ages. Croton bonplandianum Bail is an exotic weed of Euphorbiaceae family. It has medicinal use in folk medicinal practices. Herein, we report the antibacterial activities of the leaf extracts of croton against some antibiotic resistance bacteria and have isolated stigmasterol as the active component. Structure of stigmasterol was established from detail spectral analyses (IR, NMR, etc.). The leaf extracts showed promising antibacterial activities and could be a suitable alternative against antibiotic resistance bacteria.