Sanchezia speciosa is an evergreen shrub in the dogbane family Apocynaceae and planted throughout the tropical and subtropical region. It has been showed that Sanchezia speciosa has high amount of cardiac glycoside, flavonoids compounds and also the antioxidant, anti-inflammatory and anticancer activities. In this study, we aim to study the phytochemical component of ethanolic extract of Sanchezia speciosa leaf and investigate the antiinflammatory effect of Sanchezia speciosa leaf extract. Leaf of Sanchezia Speciosa Leonard was extracted with 80% ethanol. Compounds were isolated using on silica gel normal and reverse phase and preparative glassbacked TLC plates. The compound's structure were characterized on the basis of spectroscopic data, including IR, MS and NMR and by comparing their physicochemical and spectral data with those published in literatures. Paw edema was induced in the mice using 0.05 ml of 1% Carrageenan sodium salt to investigate the antiinflammatory effect of Sanchezia speciosa leaf extract. From ethanolic extract of Sanchezia speciosa leaf we have isolated four compounds. Their structures were identified as (1) Quercetin 3-O-α-L-rhamnopyranosid (quercitrin), (2) Quercetin 3-O-β-D-galactopyranosid (hyperosid), (3) sitosterol-3-O-β-D-glucopyranosid (daucosterol), (4) 3- Methyl-1H-benz[f]indole-4,9-dione. Our data showed that S.specsiosa leaves extract (dose 1.5 g/kg b.w) reduced significantly paw edema induced by Carrageenan. This is first time compound (1), (2), (4) were isolated from leaves of Sanchezia speciosa. S.specsiosa leaf extract have been showed strong effect in inhibit carrageenan-induced paw edema in mice.