Natural products from marine algae have attracted the attention of biologists and chemists the world over for the last five decades. Many of these compounds are used to treat diseases like cancer, acquired immune-deficiency syndrome, inflammation, pain, arthritis, as well as viral, bacterial, and fungal infections. The marine red alga showed the phytochemical constituents like phenols, alkaloids, saponins, flavonoids and steroids. The presence of a variety of chemical constituents, such as saponins, phenols, flavonoids, alkaloids and steroids were analyzed in Acanthophora spicifera by qualitative tests, TLC and antioxidant activities by DPPH assay .The aim of the present study is to evaluate the phytochemical constituents of the marine red alga Acanthophora spicifera.