The phytochemical constituents and antimicrobial properties of methanol, chloroform and petroleum ether extracts of flower of Cassia roxburghii were evaluated. The extract was subjected to qualitative phytochemical screening of active chemical constituents and agar well diffusion method to determine the antimicrobial properties. The results revealed the presence of saponin, flavonoids, tannins and thiols in methanol extract, phlobatannins and carbohydrates in chloroform extract and glycosides in petroleum ether extract. The methanol, chloroform and petroleum ether extracts of flowers of Cassia roxburghii were evaluated for potential antimicrobial activity against bacterial and fungal strains. The fungal isolates tested include: Epidermatophyton floccosum, Microsporum gypseum and Trichophyton mentagrophytes and the bacterial isolates tested include: Escherichia coli, Staphylococcus aureus and Vibrio sp. The zone of inhibition was measured and tabulated.