The Hemidesmus indicus is used to cure leprosy, leucoderma, itching, skin disease, asthma, bronchitis, leucorrhoea, dysentery, piles, syphilis, and paralysis. The present study was aimed to investigate the Physico-chemical and preliminary phytochemical screening of Hemidesmus indicus root. The physicochemical parameters of Hemidesmus indicus root powder were determined like extractive value, loss on drying and foaming index. The root extracts of Hemidesmus indicus were prepared using different solvents like petroleum ether, ethanol and distilled water. The phytochemical screening of the root extracts was performed. The thin layer chromatography (TLC) of ethanol and aqueous extracts were studied. The presence of alkaloids, glycosides, carbohydrates, steroids, polyphenol, saponins and terpenoids were indicated by the test conducted. The three Rf value (0.78, 0.71 & 0.38) and two Rf value (0.75 & 0.51) were found in TLC plate of ethanol and aqueous extracts respectively. These studies provided referential information in regard to its identification parameters assumed significantly in the way of acceptability of herbal drugs in present scenario of lack of regulatory laws to control quality of herbal drugs.