A major route for biological hydrogen production is purple non sulphur photosynthetic bacteria. In this study, we have analyzed the purple photosynthetic bacterial consortium isolated from Bhima amarja river of Karnataka. PNSB (Purple non sulphur photosynthetic bacteria) metabolize many different organic compounds as carbon sources and produce hydrogen. In presence of acetate, 2.0 ml of hydrogen was produced followed by cellobiose and lactate as carbon sources. Lowest amount of hydrogen was produced in benzoate and mannitol containing medium. In the presence of sodium nitrate as nitrogen source, more amounts of hydrogen was seen compared to other nitrogen sources. Ammonium chloride induced lowest amounts of hydrogen production by the photosynthetic consortium. In presence of cyanacobalamine as growth factor higher amounts of hydrogen was produced. Niacin induced lowest amounts of hydrogen. T-Test was done on two samples assuming unequal variances and the results are presented.