Solanum trilobatum is a traditional herb used widely in siddha system of medication for several ailments. Drug is being taken as powder, decoction and electuary. However characterization of the drug components would facilitate in better drug formulation strategies. Macroscopic analysis of leaves of S. trilobatum was performed to facilitate distinct identification of species. Pharmacognostical evaluation of leaves showed total ash content of 17.62 percentage; which comprises of 10.81 percentage water soluble and 6.81 percentage acid soluble ash. Also extractive value of drug powder was evaluated and results showed that alcohol soluble compounds were 3. 83 percentage and water soluble compounds were 11.24 percentage. Fluorescence analysis also was performed for drug powder using standard reagents and solvents, the results obtained were compared with established standards to indicate presence of various phyto-chemicals. Thus the above study gives the basic solubility composition and purity of drug compounds in the sample based on which drug formulations can be made for effective action.