Hemidesmus indicus (Linn.) R.Br. Family: Asclepiadaceae, is commonly known as ‘Indian sarasaparilla’. A perennial climber and growing widely in upper Gangetic plains and eastwards of Bengal and from central to southIndia. It is traditionally used for curing various ailments like stomach pain, fever, veneral disease, rheumatism and also act as a blood purifier. This traditionally useful part was standardized based on the macroscopic, microscopic, physico-chemical and chromatographic. And also estimated for its microbial limits ,pesticides, Aflatoxin and heavy metals. Preliminary phytochemical screening confirms the presence of sapnonins, tannins, resins, essential oils, sterols and glycosides determination of total saponin content.