The present study pertains to pharmacognostical, phytochemical and toxicological investigation of the medicinal important plant C. microphyllus (stem). Standardization was carried out in terms of macroscopical, microscopical characters, toxicological parameters and chromatographic analysis of C. microphyllus (stem).Different standard methods were adopted to carry out the investigation. The study will provide referential information for the correct identification of the crude drug. Foreign organic matter, extractive value, ash value, insoluble ash and loss on drying were found to be 0.0257%w/w, 15.12%w/w, 11.1%w/w, 4.92%w/w and7.26%w/w. The heavy metal analysis of arsenic was found to be 0.12 ppm, Lead 0.39 ppm, Mercury 0.01 ppm, Cadmium 0.02 ppm. These physicochemical data and phytochemical analysis of Convolvulus microphyllus is useful for further studies for pharmacological screening. In future this study will be helpful for qualitative & quantitative analysis of phytoconstituents for isolation of newer molecule.