Enterocin are a wide group of bacteriocin produced by enterococcus spp, many enterocin show bactericidal activity against pathogens such as gram positive and gram negative microorganisms. A bacteriocin producing strains were isolated from the animal feed samples of maize, rice husk, spelt, oil cake, peanuts. They have maximum production of bacteriocin was optimized at 37°C for 36hrs and at a pH of 7.0. The partial separation of enterocin was performed by precipitation with ammonium sulphate and subsequent ion exchange chromatography followed high pressure liquid chromatography. In the current work an enterocin termed as SN11 was been identified with the molecular weight of enterocin SN11 >5kDa, and it belongs class II bacteriocin of enteriocin. In the present study it is focused that purified enterocin SN11is used for food bio preservation, to prevent urinary infection, vaginal and gastro intestinal infection causing bacterium. It displayed excellent antibacterial activity against six different pathogens. Hence, the study concludes that enterocin SN11 consist of peptides, which intracts into the target cell membrane and leads to pores formation and finally induces cell death.