The aim of this study was to compare the effect of endurance training and oxidative stress reaction in men professional bodybuilders of Tehran city with traditional and cluster loading. For this purpose, 40 men aged 21.5 - 27.5 years were assigned to the following two training groups. They took part in this survey voluntary who never has had any especial diseases, metabolic or genetic problems and they had for 1month special exercises and far of smoking, drugs and doping by the uses of traditional and cluster protocols on plasma 8-OHdG and 4-HNE. For analyzing data, t-tests for independent and dependent samples were used. The results showed a significant difference between the effects of traditional and cluster protocols on plasma 8-OHdG and 4-HNE concentration. Thus, resistance exercise with traditional and cluster loading has a significant effect on the blood markers of oxidative stress, but the effect of cluster loading is significantly more great and it should be used carefully.