To study the oxidative damage prevention of DNA using passion fruit extract in earthworm as model organism. In the present study, healthy and young earthworms were collected from Tamil Nadu of Thiruvannamali district. The earthworms was packed in a sterilized bag along with soil and quickly taken to the laboratory. Based on the morphological observation, collected animals were identified as Perionyx ceylanensis with the help of zoological standard manual. The animals were then randomly divided into 3 groups of 6 animals each, placed into cages. The check the potential of passion fruit, extract was subjected to antioxidant activity. The obtained results exhibited the significant effect. The first group was given regular soil (control).The second group A was treated parotta flour with soil in 60:40 ratios (Soil: Parotta flour). Then after one week of incubation the animals were dissected and DNA was isolated to check the DNA damage in earthworm by DNA fragmentation assay. After confirmation of DNA damage, junk food treated three animals were shifted to 3rd group B from group A. Then it was fed passion fruit extract with soil in 60:40 ratios (Soil: Passion fruit extract). Then after one week of incubation the animal were dissected and DNA was isolated to check the DNA damage in earthworm by DNA fragmentation assay. This assay conformed that the passion fruit extract having the ability to repair the DNA damage which induced by junk food