In oral glucose tolerance tests with methanolic extract of Trichosanthes dioica fruits (METD), the extract dosedependently reduced blood glucose concentrations in glucose-loaded mice. At extract doses of 50, 100, 200 and 400 mg/kg, the reductions in blood glucose levels were, respectively, 2.9, 21.5, 39.8, and 46.4%. In comparison, a standard antihyperglycemic drug, glibenclamide, when administered at a dose of 10 mg per kg, reduced blood glucose level by 47.4%. In analgesic activity tests with acetic acid induced pain model mice, the extract at the aforementioned four doses, dose-dependently reduced acetic acid induced abdominal constrictions in mice by 18.5, 33.3, 37.0, and 40.7% versus the 48.1 and 63.0% reductions obtained with a standard analgesic drug, aspirin, administered respectively, at doses of 200 and 400 mg per kg. The extract when administered to mice did not cause any acute toxicity when administered at doses up to 3000 mg per kg.