Purple Non-Sulphur bacteria were isolated from tannery effluents. Among these two strains, Rhodobacter capsulatus KU002 and Rhodopseudomonas palustris KU003 were studied for the production of Polyhydroxybutyrate (PHB). Effect on different carbon and nitrogen sources on the production of PHB were tested and PHB accumulation was more in Glutamate, Malate and Yeast extract (GMY) medium in Rb.capsulatus whereas, Rps. palustris preferred succinate and Yeast extract (SY) medium. The maximum PHB produced was 313 mg/L for Rb. capsulatus and 242 mg/L for Rps. palustris. Rb. capsulatus was comparatively superior than Rps. palustris for the production of bioplastic and can be economically exploited for industrial production