A series of novel 2-[2-Amino-4(4-bromophenyl)-6H-1,3-oxazine-6-yl]-4-{3-[2-amino-4(4-bromophenyl)-6H-1,3- oxazine-6-yl]-4-hydroxybenzyl}phenol derivatives [3a-3i] were prepared from Bis[3-[(E)-3(4-bromophenyl)-3-oxo- 1-propenyl]-4-hydroxyphenyl]methane [2a-2i] with urea and potassium hydroxide in ethanol. All synthesized compounds were characterized on the basis of IR,NMR spectroscopic data and Elemental Analysis. Antimicrobial activity were evaluated and compared with the standard drugs, some compounds of the series exhibited promising anti- bacterial and anti- fungal activity compared to standard drugs.