It is noteworthy that the medications isolated directly or indirectly from phytogenic products until the recent time play a primary part in the discovery of drugs. The huge utilizations of antimicrobial agents in medicine have caused directly the development of antibiotics resistant pathogens in various infectious diseases areas, urging the detection for new and effective antimicrobial drugs. This study will be the first of its kind which is designed to evaluate antibacterial and antifungal activities and to estimate exhaustive extraction yields of the aqueous and organic extracts of Eryngium campestre L. plant. Extraction yields estimated by using serial exhaustive extraction non thermo-reactive procedures and well diffusion method were used to evaluate antibacterial and antifungal activities of aqueous and organic extracts of the Field Eryngo entire plant (E. campestre) while the minimum inhibitory concentration and minimum bactericidal-fungicidal concentration were determined by the serial dilution method. The aqueous extract showed antimicrobial activity by using well diffusion method against all gram-positive bacteria with the greatest activity against Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis. In addition to that the results showed antibacterial activity against one of the gram-negative (Pseudomonas aeruginosa) and against Candida albicans a fungi. The entire Eryngium campestre L. plant has antimicrobial activity, and we recommend exhaustive serial extraction method to get high concentration of the phytochemical yields with potential activity.