Drug delivery system in administration of pharmaceutical compounds enabled localisation of drug to the diseased site. There are different types of drug delivery system utilising immunoglobulin, serum protein, synthetic polymer, liposome, microsphere and others. Vesicular system of niosome with bilayer structure assembled by non-ionic surfactants is able to enhance bioavailability of a drug to a predetermined area for a period of time. The amphiphillic nature of noisome promotes its efficiency in encapsulating lipophilic or hydrophilic drugs. Other additives such as cholesterol can be used to maintain the rigidity of the niosome’s structure. This review describes the basic aspects of noisome include its structural components, preparation methods, limitations and current applications in various diseases.