Linezolid is an antibacterial used for the treatment of serious infections caused by Gram-positive bacteria that are resistant to several other antibiotics. Three simple, rapid and sensitive zero, first and second derivative spectrophotometric methods are developed for the determination of Linezolid in pharmaceutical dosage forms. The absorption maxima was found to be at 250 nm in Method A (D0) and shows linearity over the concentration range of 0.1-75 μg ml-1 with regression equation 0.0516x + 0.0013 (r2 = 0.999). In Method B (D1) the amplitude was chosen (240 -262 nm) and in Method C (D2) the minima (251 nm) was chosen for spectral calculations. Linezolid follows Beer-Lambert’s law over the concentration range of 0.1-60 μg ml-1 (r2= 0.999) in first order (D1) and 0.1-75 μg ml-1 (r2= 0.999) in second order derivative spectroscopy respectively. The proposed methods can be successfully applied for the determination of Linezolid in pharmaceutical formulations and validated according to ICH guidelines