Natural plants exhibit different activities due to different biologically active constituents that’s why it is the need that isolated compound fr om the plant must be fully characterized in a biological, pharmacological sense and biochemical sense. Swertia chirayita is a well known traditional Ayurvedic plant and is considered most important for its medicinal properties. It has been shown by the literature that plant are the rich sources of informative molecules like xanthones, seco-irridoid glycosides etc, main aim o f this research is to extract, isolate and characterized the active principles mainly novel co mpounds and do their biological activity two compounds have been isolated which have not bee n reported yet and their pharmacological studies (CNS) has been performed.