The present work is a computational approach which predicts potential anti hepatitis C activity of flavonoids by inhibiting NS5B polymerase of HCV virus in silico. PDB ID 3D5M was selected for docking of flavonoids against HCV NS5B polymerase as target in silico analysis. Currently there are very few antiviral therapies available for hepatitis C. Thus there is a scope to develop new drugs for treating hepatitis C. Molecular modeling approach was used to determine the potency of some naturally occurring and virtually designed flavonoid moieties against hepatitis C. Using glide software and ribavirin as a standard drug, excellent docking scores were achieved suggesting drug like characteristics of flavonoids against hepatitis C virus NS5B polymerase.