The wound healing is one of a dynamic process, which aims to re-establish the damage tissues by the process known as cytokines, macrophages and fibroblasts. The use of various bio polymeric fibers such as chitin and chitosan, alginic acid, hyaluronic acid, cellulose etc. are used for their application in soft tissue engineering, vascular tissue engineering and neural engineering. These biopolymeric fibers are worth considered as it results in a completely biodegradable composite and ensure minimum disturbance of the wound to avoid further tissue damage. These fibers are used for wound healing because of their properties like good fluid uptake for absorption of excess of exudate and blood at wound site, provide moist wound environment, better gaseous exchange and prevent from infection from microorganisms and can modify to mimic the skin environment, conditions and structure. The biopolymeric fibers enhanced wound healing by modulation of various cells, cytokines and growth factors during different phases of healing process like inflammatory, proliferative and remodeling phase. Various fabrication methods used for the preparation of fibers includes electrospinning, phase separation, self-assembly, wet spinning and template synthesis method. This review outlines the process involved in the preparation of fibers, its characterization, process involved in skin regeneration and care required for wound treatment.