The main purpose of this research was to discuss about the optimization of spray characteristics of biodiesel fuel in a spray chamber. Three factors fuel injection pressure, fuel temperature and fuel blends were chosen as the influencing factor for the set objective. Three levels were chosen in each factor and spray tip penetration (S), spray angle (θ) and Sauter mean diameter (SMD) were taken as the response variables. Experiments were designed by employing Design of experiment method and Taguchi full factorial array was used to conduct the tests with different levels of the chosen factors. Multi response Signal to Noise ratio (MRSN) was calculated for the response variables and the optimum combination level of factors was obtained simultaneously using Taguchi’s parametric design.