Azabicycle ABN (2,4-Diaryl-6,7-benzo-3-azabicyclo[3.3.1]nonan-9-ones) is a well-documented alkaloid for its wide range of biological applications and as a building block for various drug molecules. For our studies Azabicycle derivatives (5a, 5b and 5d) have been synthesized based on mannich approach, where5a is methyl, 5b is methoxy and 5d is fluoro substituted derivatives. The main aim of this study is to characterise the interaction between Bovine serum albumin (BSA) with azabicycle derivatives. BSA is used since albumin is the copious protein found in blood plasma and commonly used to investigate interaction studies of various drug molecules, so far nanoparticles like silver, iron, zinc, titanium dioxide nanoparticles etc., have been studied and some chemically synthesized metal complexed drug molecules has been established. In this study, we analyse the interaction of various concentration (6.25, 12.5, 25 and 50 μM) of azabicycle derivatives with BSA. To facilitate the preclinical development further, the interaction between ABN and BSA was studied using UV spectroscopy, Dynamic light scattering (DLS) fluorescence quenching and Fourier transform infrared spectroscopy (FTIR). The excitation wavelength 280nm of UV spectroscopy shows the interaction of compound. In DLS average size of the particles increased when interacted with BSA. FTIR peaks further confirm the interaction of ABN-BSA complex. The excitation wavelength of quenching analysis of ABN-BSA compound proves the interaction. Hence BSA has significantly decreased when increase in concentration of ABN compounds. This study reveals the detailed evaluation of ABN-BSA complex formation.