The research was focused on validation of unstructured, mathematical, kinetic model data obtained in batch shakeflask studies of Pullulan produced by Aureobasidium pullulans MTCC 2195. Logistic (L), Logistic Incorporated Leudeking-Piret (LILP) and Logistic Incorporated Modified Leudeking-Piret (LIMLP) models were used for kinetic parameters estimation. Very good fit of the data was observed between the experimental data and modelled data in biomass growth, substrate consumption and product formation kinetics. Estimated kinetic parameters like μmax, X0, Xm, α, β, γ, and η, were compared for initial Jaggery and Sucrose (as substrate) concentrations of 50, 75 and 100 g/L. Furthermore, all these parameters were predicted accurately with reasonable R2 values