The mixed ligand chelates of Cu(II), Co(II), Mn(II) and Ni(II) derived from L1 (4- chloroaniline & salicylaldehyde), L2(benzaldehyde & 2-aminophenol) and L3(4-nitroaniline & 2-hydroxyacetopheone) have been synthesized and investigated by different Physicochemical techniques. The resulting complexes were characterized by elemental analysis, molar conductance, IR, Electronic spectra and cyclic voltammogram. The Schiff base acts as bidentate monobasic ligand, coordinated through deprotonated phenolic oxygen and azomethine nitrogen atom. Their low electrical conductance values indicate that all the complexes are non-electrolytes. The electrochemical behavior, the anodic, cathodic potential and the number of electron transfer have been calculated using cyclic voltammogram. The synthesized chelates were assayed for antibacterial activity against some pathogenic bacteria: Staphylococcus aureus, Escherichia coli, Klebsilla pneumonia and Bacillus subtilis using paper disc method and placing the chelates on surface at nutrient agar. The synthesized Schiff base complexes exhibit higher antimicrobial activity than the free Schiff base because of chelation.