Interest in the chemistry of quinolines and morpholines is unabated. Quinoline, morpholine and chalcone derivatives exhibit extensive biological and pharmacological activities. Therefore some novel morpholine ring containing quinolinolyl chalcones were synthesized from 2-morpholinoquinoline-3-carbaldehyde (2) and aryl methyl ketones (3a-j) to produce (E)-3-(2-morpholinoquinolin-3-yl)-1-aryl prop-2-en-1-ones (4a-j) by conventional and non-conventional methods. The newly synthesized compounds were characterized using Mass Spectrometry, IR, 1 H-NMR, 13C-NMR, and Elemental analysis. These compounds were evaluated for their antibacterial activity against gram positive and gram negative strains using a micro dilution procedure. Synthesized compounds showed activity against a panel of microorganisms.