Measurements of physicochemical properties were carried out for understanding the behavior of mixed surfactant system containing novel hydroxylated gemini surfactant Butanediyl-1,4-bis(hexadecyl hydroxyethyl methyl ammonium bromide) 16-4-16 MEA (where MEA is monoethanol amine) and its corresponding monomeric counterpart cetyl dimethylethanol ammonium bromide (C16DMEAB). The Kraft temperature (kT), critical micelle concentration (CMC) and average degree of micelle ionization (α) of mixed micelle was determined from conductance data. CMC and α values were observed to decrease significantly with increasing mole fraction of 16-4- 16 MEA surfactant in mixed micellar system. The Kraft temperature (kT), methyl methacrylate oil (MMA) solubilization capacity, foamability and foam stability of mixed surfactant systems were also examined.