A simple precise, accurate Normal phase High performance liquid chromatographic method has been developed for the Determination Of Enantiomeric Purity of Levobetaxolol by Chiral Chromatography. In this method a Chiral OD-H 25 cm x 4.6 mm x 5.0μ (Cellulose based) column with mobile phase Hexane: Ethanol: Diethyl amine (95:5:0.1v/v),was used. The detection wavelength is 220 nm and the flow rate is 1.0 ml/min. The linearity of Levobetaxolol shows regration coefficient of 0.9999. The proposed method is sufficiently selective to distinguish the parent drugs from its other enanatiomer.