In Brazil, there is an epidemiological crisis scenario in relation to arboviruses like dengue, Chikungunya, and Zica, where all have in common vector, Aedes aegypti. The species Pseudoxandra cuspidate Maas (1983), is from Annonaceae family, popularly known as Lamuci, Envira, Amarela, Envira Preta, Envira Lamuci, and is referred to by traditional medicine with anti-inflammatory, antifungal and antimalarial; It is endemic in the north, but little studied in relation to its biological potential and its chemical constituents. The Pseudoxandra cuspidate following the chemotaxonomic profile of the Annonaceae family is rich in alkaloids. Based on these assumptions, this study has to analyze the potential larvicide with Aedes aegypti and antioxidant activity of the crude ethanol extract of P. cuspidata. In relation to the antioxidant activity of the crude ethanolic extract, IC50% = 326.85 μg/ml and in relation to larvicidal activity against Aedes aegypti intermediate stage of LC50% for 24 hours was 437.79 ppm for 48 hours and concentration was 270.85 ppm. In both activities, the result was significant, starting new possibilities of using the ethanol crude extract Pseudoxandra cuspidata.