The Raman Effect occurs when light impinges upon a molecule and interacts with the electron cloud and the bonds of that molecule. Raman spectro scopy is commonly used in chemistry, since vibrational information is specific to the chemical bonds and symmetry of molecules. It therefore provides a fingerprint by which the molecule can be identified.A new spectroscopy technique to uncover the precise sequence of atomic movements an d structural changes that occur during complex chemical transformations. GFP involves a cl assic intermolecular proton transfer reaction to produce its fluorescent form. This happ ens on an ultra fast timescale - from 10 femtoseconds to 10 picoseconds. To perform structur al measurements and determine the mechanism and pathway of any reaction occurring in this time range by using femtosecond stimulated Raman spectroscopy (FSRS). A great deal was known about the kinetics of the proton transfer reaction but little was known about the ac tual complexity of the reaction coordinate or combination of atomic motions that must occur befor e the proton can be transferred. The time resolved structural data which told us the actual a tomic motions that are necessary. 'This work shows a possibility that we will be able to know ho w structure changes in real-time even in very complex molecules like a protein using FSRS. _______________________________________