Isozymic variation studies on Tectaria coadunata (J. Smith) C.Chr., Tectaria wightii (Clarke) Ching and Tectaria paradoxa (Fee) Sledge was carried out to reveal the diversity existing at molecular level. PAGE was employed for the isoperoxidase analysis. A total of seventeen bands with five active regions were expressed in this enzyme system. Present study revealed hundred percentage genetic differentiations between the three Tectaria species. The isoperoxidase banding profiles confirmed the classification based on morphology. The present study confirms that all the three species are morphologically and genetically distinct, but cytologically uniform.