Quarries which are located close to surface water or ground water and the engineering people often facing the problems with drainage. Pseudomonas aeruginosa, can be used for biodegradation in liquid culture, but the roles and production in soils are unknown. Surface-active compounds for bacterial production like biosurfactants and bioemulsifiers which will increase the hydrocarbons of local pseudosolubility and that will improve in mass transfer to biodegrading bacteria. Several strains of potassium solubilizing bacteria, such as Acidothiobacillus ferroxidans, Bacillus mucilogenous, Pseudomonas, Burkholderia, B. edaphicus and B. circulans can be used as biofertilizer. Hence this study is focused on isolation and identification of Pseudomonas aeruginosa from quarry sand and it will be used further for more application purposes.