Xylanases are important class of hydrolytic enzyme involved in degradation of xylan- a back bone of hemicellulose. It has wide applications in biotechnological processes such as food, feed, baking, pulp and paper, bio fuel, fruit waste treatment. In the present investigation xylanase producing fungi were screened from the samples collected from various natural habitats such as forest, farm soil and compost pile. Plate assay technique with basal media containing 0.5 % [w/v] beech wood xylan was used for the primary screening. Thirty one fungal strains were obtained though this screening which has xylanase production ability. The fungal isolate MR 57 has showed highest potential in xylanase production with 4745.45 U/ml after 6th day of incubation using submerged fermentation. The isolate was further identified as Aspergillus heteromorphus using morphological as well as molecular approach and sequence was submitted to gene bank.