The present study has been carried out to evaluate the microorganisms from goat intestine. The microorganisms were isolated by streak plate method, and the microorganisms were identified by the serial dilution method. The components present in the goat intestine were detected by biochemical test such as indole, methyl red, vogesproskauer’s test, mannitol test, urease test, triple sugar ion test, sucrose test and glucose test. The microorganisms were screened under microscope for its structural elucidation.