In the ethno-pharmacological approach, local knowledge about the potential uses of the plants is very useful as compared to the random approach where indigenous knowledge is not taken into consideration. Compounds for different pharmacological activities have been isolated from plants. Plants are capable of synthesizing an overwhelming variety of low molecular weight organic compounds usually unique and complex in structure. Phytochemicals produced in plants are secondary compounds responsible metabolic activities and defense in purpose. In the present investigation, a significant biomarker, amarogentin was isolated from the gradient column fractionation of hydro-alcoholic extract of Swertia chirayta. The compound was obtained as a pale yellow powder after drying and was found to be an iridoid glycoside. The compound showed Rf value of 0.75 when compared to standard. The isolated compound showed the retention time of 6.78 minutes very much similar to that of the standard. The compound demonstrated paralysis as well as death of worms in a lesser time at 20 μg/ml compared to piperazine citrate and albendazole (15 mg/ml). The results showed the significant potency of amarogentin as a significant anti-helminthic.