Probiotics are living micro organism used as food supplements, which provide health benefit when consumed, by improving intestinal microbial balance of host. Probiotics have high antagonistic activity, antimicrobial activity. The probiotic strain Lactobacillus casei, Lactobacillus rahmnosus, Lactobacillus fermentum and were used as pure culture and standard strains. Probiotics reduce the risk of intestinal disease like diarrhea and typhoid, probably due to their role in suppressing the activity of certain bacterial enzymes with the production of bacteriocins or with the help of lowering the pH by producing certain acids like lactic acid and hydrogen peroxide. Thus probiotic treatment will offer a promising alternative to the use of antibiotics in healthcare.