n-hexadecanoic acid ( Palmitic acid) was isolated from Canthium parviflorum leaves extract. The isolated compound was characterized by FTIR and MS. Further, the antimicrobial activity of ethanolic extract of C. parviflorum leaves was screened against Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis), and fungi (Candida albicans).