This research aimed to isolation and characterization β-amyrin palmitate (olean-12-en 3β-ol hexadecanoate) from fruit of Ficus aurata (Miq.) Miq.Separation of compounds were done by column chromatography and thin layer chromatography. The presence of this compound was reported for the first time from this medicinal plant. The structure of this compound was characterized based on spectroscopic analysis and comparison with published in the literature.