Toona Sureni (blume) Merr is one of species in the Meliaceae family. Phytochemical screening of the bark of Toona Sureni showed the presence of flavonoids, phenolics, tannins, terpenoids and saponins. According to preliminary examination, this research has been conducted on isolation and characterization flavonoids from the bark of Toona sureni. Isolation was done by macerated with n-hexane, ethyl acetate and methanol. Each of extracts was eluted in column chromatography (silica gel) to get a pure extract. Based on spectrum analysis of UV-vis, IR, NMR and mass spectroscopy were known that flavonoids compound in ethyl acetate extract was catechin.