The tanning industry was designated as “Red category” due to the high pollution contributed to environment by its solid and liquid wastes the toxicity by heavy metals is spreading throughout the world along with industrial progress. The present study deals with isolation, identification and characterization of isolated from tannery effluent collected in and around Erode, South India. A total of 60 isolates were screened from tannery effluent. Antibiotic sensitivity pattern was studied using disk diffusion method. Most of the bacterial strains were sensitive sensitive to cotrimoxazole, gentamycin, kanamycin, nalidixic acid, ampicillin, and Penicillin showed resistance against bacteria. Staph aureus was 50% resistant to amikacin, 49% resistant to gentamycin and norfloxacin. Bacillus spp. demonstrated higher resistance for Ciprofloxacin similarly E.coli had 49.7% resistance to clindamycin. P.aeruginosa exhibited very high resistance for colistin and norfloxacin. The two major dominant isolates were Pseudomonas spp. and Bacillus spp., Staph aureus was found to be in subdominant form. It is found that domestic and industrial effluents are responsible for the development of bacterial resistance with the risk of human health and environment.