The present study was designed to investigate in-vitro hepatoprotective activity of Moringa oleifera mediated synthesis of gold nanoparticle. The gold nanoparticles (GNPs) were synthesised from pods of Moringa oleifera. Aqueous, ethanolic and chloroform extracts have been screened for the suitability of nanoparticles synthesis. Phytochemical screening was performed to identify the major secondary metabolites and its antibacterial efficacy towards Bacillus subtilis, Escherichia coli, Klebsiella pneumonia and Staphylococcus aureus were investigated. The pod mediated synthesis of gold nanoparticles were characterised by Ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). Further, in-vitro hepatoprotective activity of gold nanoparticleis carried out by HEP G2 cell line (liver hepato-cellular carcinoma). Chloroauric acid when exposed to pod extract was reduced and converted to gold nanoparticles. The phytochemical screening indicated the presence of alkaloids, terpenes, phenol and tannin, saponins, flavonoids, quinines and proteins. The antibacterial efficacy proved that Moringa oleifera could be used in the treatment of infection caused by such pathogens as S. aureus, E. coli, B. Subtilis and K. pneumoniae. The ultraviolet-visible spectrum of the gold nanoparticles showed an absorption peak at around 559 nm, the scanning electron microscopy indicated that the average size of gold nano particle was about 40 - 80nm, X-ray diffraction showed that the particles were crystalline in nature, FTIR showed the presence of functional group involved in the reduction, stabilization and capping of the GNPs. From the tryphan blue assay, gold nano particle showed greater activity on HEP G2 cell line.