In vitro antioxidant property and antibacterial assay of the three genders of minor millets (Echinochloa crus-galli, Panicum miliaceum L, Panicum sumatrense) were evaluated. IC50 for various extracts were determined for DPPH scavenging, bleaching of b- carotene and % inhibition of H2O2 and compared with standard positive controls viz. butylated hydroxytoluene (BHT) for DPPH, propyl gallate for b-carotene and ascorbic acid for H2O2 assay. Antibacterial assay of six extracts of three species were evaluated against bacterial strains of Staphylococcus aureus (MTCC 96), Bacillus megaterium (MTCC-428), Escherichia coli (MTCC 443) and Pseudomonas aeruginos (MTCC1688). It is concluded from the results that phytochemicals are responsible for such inhibition of multi resistance microorganisms and could be a source of new antibacterial drugs.