Parvovirus B19 infects especially thalassemia and sickle cell anemia patients .In these Patients, the production of precursors of red blood cells increases to compensate the dissolution of red blood cells. B19 infections lead to suppression of the erythrocytes formation and acute erythroblastopenia often called transient aplasia crisis which may be life-threatening. These patients usually become susceptible to dramatically viral infection and increased risk of transmission viral infection. This study included 200 patients with Haemoglobinopathy (101 male, 99 female). Specimens were collected randomly (100 Sickle cell anemia and 100 Thalassemia) from thalassemia center in Damascus-Syria between November 2014 and January 2015.B19V DNA were detected by NESTED PCR in sera.Of the 200 patients,18 (9%) were seropositive for B19DNA.This study showed that there was relationship between seropositive and age, but no relationship between B19 infection and gender .