Nowadays as the result of environmental conditions like fast foods and stress, colon cancer is becoming common. Different treatments are presented for cancer disease. Chemotherapy and radiotherapy are the main parts of cancer therapy and different drugs are also used for increasing immune system and specifically targeting cancer cells. Natural methods for treating human disease are becoming popular because of their low side effects and good results. Bee products are probably one of the first things used for treating human disease. The anti-proliferative properties of bee venom investigated against different types of cells. In this study the effect of Honey bee venom investigated against colon cancer cells HT-29 and compared with normal cells L929.