Phytophthora capsici causes devastating disease on many crop species, including capsicum. Resistance in capsicum annuum is genetically and physiologically complex. ‘Criollo de Morelos 334’ (CM334) from America is one of the most promising sources of resistance to Phytophthora capsici in pepper. In this work, using the traditional genetic analysis method, a joint analysis of six-generations (P1, P2, F1, F2, BC1 and BC2) was performed from cross between CM334 and a highly susceptible pepper line 949. The resistance of plants of each population was evaluated by irrigating method. F2 population was analysed with SRAP molecular markers. Results showed that a single pair of nuclear and dominant gene controlled the resistance in CM334. Among 64 pairs of SRAP primers, 21 pairs of primers produced polymorphic bands in parents. After screening of the F2 population using the 21 pairs of SRAP primers, we obtained a SRAP marker linked with Phytophthora blight resistance, designated SRAP- Me6/Em15.