The aim of the present study was to assess the Pharmacognostic and Phytochemical studies on Leaves of Stephania japonica Linn. The objective of the present work comprise of collection, identification, microscopical and phytochemical evaluation of Leaves of Stephania japonica Linn. The leaves section were taken and cellular structures were studied. The T.S of Leaves shows the presence of epidermis, vascular bundle, veinislet, veintermination, spongy mesophyll, xylem, phloem,sclerenchyma,glandular and non glandular trichomes. The phytochemical studies indicate the Ash value, Extractive value, Crude fibre content, Fluorescence characters and the Preliminary phytochemical tests on various extracts indicates the presence of alkaloids, glycosides, flavanoids, saponins, carbohydrates, tannins, phenols, and mucilage.