Effect of ethanolic extract of F. religiosa fruits extract was studied against two gram positive bacteria (Staphylococcus epidermidis and Staphylococcus aureus) and two gram negative bacteria(Pseudomonas vulgaris and Klebsiella pneumonia). The minimum inhibitory concentration of extract effective against S. epidermidis and K. pneumonia was 15 mg/ml while the minimum inhibitory concentration for the S. aureus and P. vulgaris was 30 mg/ml. At 15 mg/ml concentration of extract K. pneumonia showed more sensitivity (inhibition zone 21 mm) than S. epidermidis (inhibition zone 19 mm). At 30 mg/ml concentration P. vulgaris showed more sensitivity (inhibition zone 12 mm) than S. aureus (inhibition zone 9 mm).Present observations indicate that the extracts possess antibiotic activity. Further scientific evaluation is needed to screen active phytochemical from this important plant to use it for production of new antibiotics.