Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2010 Vol: 2 Issue: 5

In vitro propagation of Boerhaavia diffusa L. through direct and indirect organogenesis

Abstract

The present study was aimed to develop an efficient reproducible protocol for the large scale propagation of medicinally important Boerhaavia diffusa L using the shoot-tip, nodal, internodal and leaves as explants. Highest percentage (97.2 ± 0.68 for nodal segments and 95.6 ± 0.82 for Shoot tip) of multiple shoot proliferation (14.4 ± 0.64 for nodal segments and 7.83 ± 0.38) was observed on MS medium augmented with 1.5 mg/l of BAP. The maximum number of shootlets regeneration (11.6 ± 0.38) was observed from MS medium supplemented with 3.0 mg/l of BAP. Maximum percentage (73.2 ± 0.47) of shootlets was observed in MS medium supplemented with 3.0 mg/l of BAP. Highest percentage, maximum number of rootlets/shootlet and mean length of rootlets were observed in ½ MS medium supplemented with 1.0 mg/l of IBA.

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